Semmelweis University
Department of Medical Biochemistry
SOLID TUMORS OR HEALTHY TISSUE FOR RPPA

Ship tumors/tissues (min 20 mg, max 200 mg) following the instructions shown in 'sample submission' link, in dry ice (minimum 5 kg box). Include only one tube per tumor or healthy tissue or stroma. Do not submit multiple tubes from the same tumor or healthy tissue or stroma.
Protocols
Project Leader: Christos Chinopoulos, MD, PhD
 
CELL PELLET PREPARATION FOR RPPA

Attached cells:

1. Collect a minimum of 2 million cells by either Trypsin EDTA or scraping
2. Wash cell pellet twice with PBS (re-suspend and centrifuge, 2.5 min @3,5000 rpm)
3. Aspirate PBS as much as possible without disturbing cell pellet
4. Store dry cell pellet in -80 oC
5. Ship samples in dry ice (minimum 5 kg box)

Suspension cells:

1. Collect a minimum of 2 million cells by centrifugation (2.5 min @3,500 rpm)
2. Wash cell pellet twice with PBS (re-suspend and centrifuge)
3. Aspirate PBS as much as possible without disturbing cell pellet
4. Store dry cell pellet in -80 oC
5. Ship samples in dry ice (minimum 5 kg box)

Along with the cell pellets please send us the information requested as detailed in 'sample submission' link  in this excel sheet (tab 'Other'). We prefer to obtain this information prior to receiving the samples.
Along with the tumor/tissues please send us the information requested as detailed in 'sample submission' link  in this excel sheet (tab 'Living human subject' or 'post-mortem', depending on the origin of the sample). We prefer to obtain this information prior to receiving the samples.
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