Semmelweis University
Department of Medical Biochemistry
At the moment, we only accept samples from solid tumors (or healthy tissues) from humans or animals as well as cell culture pellets; we do not accept blood or any other fluids. If samples originate from patients that are hepatitis C- or HIV-positive (or other), please contact us for special instructions. Each sample must be submitted in a specific cryovial tube, as indicated below:
 
If sample is from a living human subject (tab "Living human subject"):

- Hungarian patient TAJ szám: (equivalent to SSN); it will be encrypted by our software

- Foreign identifier: this is an identifier for the sample (not the patient)

- Cryovial barcode: same as written on the tube; (input to the excel sheet from a barcode reader is preferred, so as to avoid typing errors)

- External reference: this is an identifier for patients who do not have a Hungarian TAJ szám, but can be identified by i.e. I.D. or similar; it has to be unique for each patient.

- For Healthy tissues, type H; for Tumor tissues, type T; for Stroma, type S (column E)

- Type of tumor (If tumor or stroma). Tumor must be classified according to the International Classification of Diseases, Tenth Revision, Clinical Modification (ICD-10-CM). A list of tumors can be downloaded here.

- Type of tissue (if healthy). Tissue must be classified according to the International Classification of Diseases for Oncology, Third Edition, First Revision: http://codes.iarc.fr/. A list of tissues can be downloaded here in csv format.

- Gender of the patient

- Date of Birth (DD/MM/YYYY)

- Date of surgery (DD/MM/YYYY)

- Tumor percentage (if tumor or stroma)

- Pathologist who classified the tumor and estimated tumor percentage in the sample (if not known, type unknown)

- Means of freezing of the sample (i.e. -20 oC, snap frozen in LN, vacuum-foiling, etc)

- Time elapsed until freezing of the sample (in minutes)

- Site of origin of the sample (Clinic or Institute from where the sample was taken)

- Comments (for anything else)
Sample submission
The type of the cryovial tube is CL2ARBIPSTS from Biosigma: Barcoded Cryotube 2 ml, internal thread cap, skirted, sterile, graduated, writing area, CLEARLINE. Barcode Code 128.
NOTE: We cannot process FFPE or otherwise fixed samples. Samples must have been frozen to -20 oC or lower within 6 hours upon excision (if obtained by surgical means).
If sample is from animal origin (tab "Animal"):

- Foreign identifier: this is an identifier for the sample (not the animal)

- Cryovial barcode: same as written on the tube; (input to the excel sheet from a barcode reader is preferred, so as to avoid typing errors)

- External reference: this is an identifier for the animal, i.e. I.D. or similar; it has to be unique for that animal

- For Healthy tissues, type H; for Tumor tissues, type T; for Stroma, type S (column D)

- Type of tumor (If tumor or stroma). If the tumor is a human xenograft, it must be classified according to the International Classification of Diseases, Tenth Revision, Clinical Modification (ICD-10-CM). A list of tumors can be downloaded here.

- Type of tissue (if healthy)

- Gender of the animal

- Date of Birth (DD/MM/YYYY)

- Date of surgery or vivisection (DD/MM/YYYY)

- Tumor percentage (if tumor or stroma)

- Pathologist who classified the tumor and estimated tumor percentage in the sample (if not known, type unknown)

- Means of freezing of the sample (i.e. -20 oC, snap frozen in LN, etc)

- Time elapsed until freezing of the sample (in minutes)

- Site of origin of the sample (Clinic or Institute from where the sample was taken)

- Comments (for anything else)

- Type of animal (please include strain, if available.)

- Date of Collection (DD/MM/YYYY)
Write the foreign identifier (or the TAJ szám for Hungarian patients, see below for explanations) on the white area of the tube. Do not attach any stickers; do not cover or overwrite the barcode.
 
If sample is human post-mortem (tab "Post-mortem"):

- Foreign identifier: this is an identifier for the sample (not the cadaver)

- Cryovial barcode: same as written on the tube; (input to the excel sheet from a barcode reader is preferred, so as to avoid typing errors)

- Post-mortem reference: this is an identifier for the cadaver, i.e. I.D. or similar; it has to be unique for that cadaver

- For Healthy tissues, type H; for Tumor tissues, type T; for Stroma, type S (column D)

- Type of tumor (If tumor or stroma). Tumor must be classified according to the International Classification of Diseases, Tenth Revision, Clinical Modification (ICD-10-CM). A list of tumors can be downloaded here.

- Type of tissue (if healthy). Tissue must be classified according to the International Classification of Diseases for Oncology, Third Edition, First Revision: http://codes.iarc.fr/. A list of tissues can be downloaded here in csv format.

- Gender of the cadaver

- Date of Birth (DD/MM/YYYY)

- Tumor percentage (if tumor or stroma)

- Pathologist who classified the tumor and estimated tumor percentage in the sample (if not known, type unknown)

- Means of freezing of the sample (i.e. -20 oC, snap frozen in LN, etc)

- Time elapsed until freezing of the sample (in minutes)

- Site of origin of the sample (Clinic or Institute or Tisse Bank from where the sample was taken)

- Date of Collection (DD/MM/YYYY)

- Comments (for anything else)
If sample is 'Other' (i.e. cell culture material, tab "Other"):

- Foreign identifier:this is an identifier for the sample (not the tissue)

- Cryovial barcode: same as written on the tube; (input to the excel sheet from a barcode reader is preferred, so as to avoid typing errors)

- External reference: this is an identifier for the tissue; it has to be unique for that tissue

- For Healthy tissues, type H; for Tumor tissues, type T; for Stroma, type S (column D)

- Type of tissue (if healthy) or tumor: if cancer cell line, indicate the line. If non-tumor, specify the type of tissue (i.e. primary cell line). Please also indicate if it is cell culture pellets, isolated organelles, etc)

- Means of freezing of the sample (i.e. -20 oC, snap frozen in LN, etc)

- Time elapsed until freezing of the sample (in minutes)

- Site of origin of the sample (Clinic or Institute from where the sample was taken)

- Date of Collection: DD/MM/YYYY

- Comments (for anything else)
Ship your samples in the cryovial tubes in dry ice. Please contact us for shipping details.
Along with the sample, please provide this excel sheet with the following mandatory information:
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